The polymerase chain reaction (PCR) is a molecular method that is employed for in vitro amplification of a specific region in a strand of deoxyribonucleic acid (DNA). Multiplex PCR involves the ...

The modular multiplex RT-PCR method simultaneously detects Shiga Toxin E. coli (STEC), Salmonella spp. and Listeria monocytogenes using a single, overnight enrichment (24 hours). Ready-to-use PCR ...

“Where PCR is really going,” says Olivier Harismendy at the University of California, San Diego, “is parallelization and miniaturization.” Indeed, researchers are making use of a wide variety of ...

The changing technology landscape will affect the BioWatch program. This chapter comments on additional assay performance considerations that arise from the use of multiplexed real-time polymerase ...

Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics. As a sensitive and precise method for quantitating specific nucleic acid targets, qPCR is ...

High-throughput discovery technologies deliver an astounding quantity of data but often the greater challenge is to identify real targets from the vast body of potential hits. Typically, this task ...

There are many different technologies available to conduct multiplex PCR. However, each comes with its own sets of pros and cons. This infographic compares three pillars of PCR, quantitative PCR, ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

A team of UK-based scientists has recently developed an allele-specific probe polymerase chain reaction method that can accurately detect different variants/lineages of severe acute respiratory ...

With laboratory methods and diagnostics advancing apace, Francesca Lake considers whether PCR is likely to retain its crown as an essential lab method, or whether it could be replaced and consigned to ...